Selected Publications

Singular location and signaling profile of adenosine A2A-cannabinoid CB1 receptor heteromers in the dorsal striatum

Moreno E, Chiarlone A, Medrano M, Puigdellívol M, Bibic L, Howell LA, Resel E, Puente N, Casarejos MJ, Perucho J, Botta J, Suelves N, Ciruela F, Ginés S, Galve-Roperh I, Casadó V, Grandes P, Lutz B, Monory K, Canela EI, Lluís C, McCormick P, Guzmán M

Neuropsychopharmacology. 2018 Apr;43(5):964-977. doi: 10.1038/npp.2017.12.
Double deficiency of Trex2 and Dnase1L2 nucleases leads to accumulation of DNA in lingual cornifying keratinocytes without activation of inflammatory responses

Manils J, Fischer H, Climent J, Casas E, García-Martínez C, Bas J, Vavouri T, Ciruela F, de Anta JM, Tschachler E, Eckhart L, Soler C.

Sci Rep. 2017 Sep 19;7(1):11902. doi: 10.1038/s41598-017-12308-4
Cognitive impairments associated with alterations in synaptic proteins induced by the genetic loss of adenosine A2A receptors in mice

Moscoso-Castro M, López-Cano M, Gracia-Rubio I, Ciruela F, Valverde O.

Neuropharmacology. 2017 Aug 26;126:48-57. doi: 10.1016/j.neuropharm.2017.08.027.
The Parkinson's disease-associated GPR37 receptor interacts with striatal adenosine A2A receptor controlling its cell surface expression and function in vivo

Morató X, Luján R, López-Cano M, Gandía J, Stagljar I, Watanabe M, Cunha RA, Fernández-Dueñas V, Ciruela F.

Sci Rep. 2017 Aug 25;7(1):9452. doi: 10.1038/s41598-017-10147-x.
Bitopic fluorescent antagonists of the A2A adenosine receptor based on pyrazolo[4,3e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine functionalized congeners.

Duroux, R, Ciancetta, A, Mannes, P, Yu, J, Boyapati, S, Gizewski, E, Yous, S, Ciruela, F, Auchampach, JA, Gao, Z-G, Jacobson, KA.

Med. Chem. Commun., 2017,8, 1659-1667. doi: 10.1039/C7MD00247E
Locus coeruleus at asymptomatic early and middle Braak stages of neurofibrillary tangle pathology

Andrés-Benito P, Fernández-Dueñas V, Carmona M, Escobar LA, Torrejón-Escribano B, Aso E, Ciruela F, Ferrer I.

Neuropathol Appl Neurobiol. 2017 Aug;43(5):373-392. doi: 10.1111/nan.12386.
Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Morató X, López-Cano M, Canas PM, Cunha RA, Ciruela F.

J Vis Exp. 2017 May 12;(123). doi: 10.3791/55661.
Angiotensin II type 1/adenosine A 2A receptor oligomers: a novel target for tardive dyskinesia

Oliveira PA, Dalton JAR, López-Cano M, Ricarte A, Morató X, Matheus FC, Cunha AS, Müller CE, Takahashi RN, Fernández-Dueñas V, Giraldo J, Prediger RD, Ciruela F.

Sci Rep. 2017 May 12;7(1):1857. doi: 10.1038/s41598-017-02037-z.
Lighting up G protein-coupled purinergic receptors with engineered fluorescent ligands

Ciruela F, Fernández-Dueñas V, Jacobson KA.

Neuropharmacology. 2015 Apr 16; 98: 58-67. doi: 10.1016/j.neuropharm.2015.04.001
Visualizing G Protein-Coupled Receptor-Receptor Interactions in Brain Using Proximity Ligation In Situ Assay

Taura J, Fernández-Dueñas V, Ciruela F.

Curr Protoc Cell Biol. 2015 Jun 1;67:17.17.1-17.17.16 doi: 10.1002/0471143030.cb1717s67.
Untangling dopamine-adenosine receptor-receptor assembly in experimental parkinsonism in rats.

Fernández-Dueñas V, Taura JJ, Cottet M, Gómez-Soler M, López-Cano M, Ledent C, Watanabe M, Trinquet E, Pin JP, Luján R, Durroux T, Ciruela F.

Dis Model Mech. 2015 Jan;8(1):57-63. doi: 10.1242/dmm.018143.
Uncovering caffeine's adenosine A2A receptor inverse agonism in experimental parkinsonism

Fernández-Dueñas V, Gómez-Soler M, López-Cano M, Taura JJ, Ledent C, Watanabe M, Jacobson KA, Vilardaga JP, Ciruela F.

ACS Chem Biol. 2014 Nov 21;9(11):2496-501. doi: 10.1021/cb5005383.
Coassembly and coupling of SK2 channels and mGlu5 receptors

García-Negredo G, Soto D, Llorente J, Morató X, Galenkamp KM, Gómez-Soler M, Fernández-Dueñas V, Watanabe M, Adelman JP, Shigemoto R, Fukazawa Y, Luján R, Ciruela F.

J Neurosci. 2014 Oct 29;34(44):14793-802. doi: 10.1523/JNEUROSCI.2038-14.2014.
Photomodulation of G protein-coupled adenosine receptors by a novel light-switchable ligand

Bahamonde MI, Taura J, Paoletta S, Gakh AA, Chakraborty S, Hernando J, Fernández-Dueñas V, Jacobson KA, Gorostiza P, Ciruela F.

Bioconjug Chem. 2014 Oct 15;25(10):1847-54. doi: 10.1021/bc5003373.
Portraying G protein-coupled receptors with fluorescent ligands

Ciruela F, Jacobson KA, Fernández-Dueñas V.

ACS Chem Biol. 2014 Sep 19;9(9):1918-28. doi: 10.1021/cb5004042.
Prevalence, risk factors and main features of adverse drug reactions leading to hospital admission

Pedrós C, Quintana B, Rebolledo M, Porta N, Vallano A, Arnau JM.

Eur J Clin Pharmacol. 2014 Mar;70(3):361-7. doi: 10.1007/s00228-013-1630-5.
Predicting the antinociceptive efficacy of σ(1) receptor ligands by a novel receptor fluorescence resonance energy transfer (FRET) based biosensor

Gómez-Soler M, Fernández-Dueñas V, Portillo-Salido E, Pérez P, Zamanillo D, Vela JM, Burgueño J,Ciruela F.

J Med Chem. 2014 Jan 9;57(1):238-42. doi: 10.1021/jm401529t.
Targeting striatal metabotropic glutamate receptor type 5 in Parkinson's disease: bridging molecular studies and clinical trials

Vallano A, Fernandez-Duenas V, Garcia-Negredo G, Quijada MA, Simon CP, Cuffí ML, Carbonell L, Sanchez S, Arnau JM, Ciruela F.

CNS Neurol Disord Drug Targets. 2013 Dec;12(8):1128-42.
The Parkinson's disease-associated GPR37 receptor-mediated cytotoxicity is controlled by its intracellular cysteine-rich domain.

Gandía J, Fernández-Dueñas V, Morató X, Caltabiano G, González-Muñiz R, Pardo L, Stagljar I, Ciruela F.

J Neurochem. 2013 May;125(3):362-72. doi: 10.1111/jnc.12196.
Molecular determinants of A2AR-D2R allosterism: role of the intracellular loop 3 of the D2R

Fernández-Dueñas V, Gómez-Soler M, Jacobson KA, Kumar ST, Fuxe K, Borroto-Escuela DO, Ciruela F.

J Neurochem. 2012 Nov;123(3):373-84. doi: 10.1111/j.1471-4159.2012.07956.x.
Fluorescence resonance energy transfer-based technologies in the study of protein-protein interactions at the cell surface

Fernández-Dueñas V, Llorente J, Gandía J, Borroto-Escuela DO, Agnati LF, Tasca CI, Fuxe K, Ciruela F.

Methods. 2012 Aug;57(4):467-72. doi: 10.1016/j.ymeth.2012.05.007.
An update on adenosine A2A receptors as drug target in Parkinson's disease

Vallano A, Fernandez-Duenas V, Pedros C, Arnau JM, Ciruela F.

CNS Neurol Disord Drug Targets. 2011 Sep 1;10(6):659-69.
Lighting up multiprotein complexes: lessons from GPCR oligomerization

Ciruela F, Vilardaga JP, Fernández-Dueñas V.

Trends Biotechnol. 2010 Aug;28(8):407-15. doi: 10.1016/j.tibtech.2010.05.002.
Fluorescence-based methods in the study of protein-protein interactions in living cells

Ciruela F.

Curr Opin Biotechnol. 2008 Aug;19(4):338-43. doi: 10.1016/j.copbio.2008.06.003.
Detection of heteromerization of more than two proteins by sequential BRET-FRET

Carriba P, Navarro G, Ciruela F, Ferré S, Casadó V, Agnati L, Cortés A, Mallol J, Fuxe K, Canela EI, Lluís C, Franco R.

Nat Methods. 2008 Aug;5(8):727-33. doi: 10.1038/nmeth.1229.