Biotecnologia Vegetal: Producció de Fitofàrmacs

PLANT BIOTECHNOLOGY: PHYTOCHEMICAL PRODUCTION

 

INTRODUCTION

The Plant Biotechnology: Phytochemical Production research group has extensive know-how in the development of cell culture- and plant organ-based biotechnology platforms for the production of secondary metabolites of interest for the chemical-pharmaceutical industry and the production of plant stem cells used directly as cosmetic ingredients. In this context, we have collaborated with several pharmaceutical and cosmetic companies in the industrial implementation of these techniques.

 

PERSONNEL

Javier Palazon Barandela (javierpalazon@ub.edu)

Mercè Bonfill Baldrich (mbonfill@ub.edu)

Elisabet Moyano Claramunt (elisabeth.moyano@upf.edu)

Diego Alberto Hidalgo-Martínez (dhidalgo@ub.edu)

 

CURRENT RESEARCH LINES

1. Improvement of the production of taxol and other taxanes of interest by metabolic engineering of in vitro cultures of Taxus spp

2. Biotechnological production of triterpene saponines in cell and root cultures of Centella asiatica

 

PUBLICATIONS

 

Research articles (last 5 years)

 

1. Selim DH, Nassar R, Boghdady MS, Bonfill M (2019) Physiological and anatomical studies of two wheat cultivars irrigated with magnetic water under drought stress conditions. Plant Physiology and Biochemistry 135: 480-488. doi: 10.1016/j.plaphy.2018.11.012. (IF 2,718)

 

2. Khojasteh A, Sanchez-Muñoz R, Moyano E, Bonfill M, Cusido RM, Eibl R, Palazon J (2019) Biotechnological production of ruscogenins in plant cell and organ cultures of Ruscus aculeatus. Plant Physiology and Biochemistry 141: 133-141. doi: 10.1016/j.plaphy.2019.05.029. (IF 3.404)

 

3. Sanchez-Muñoz R, Almagro L, Cusido RM, Bonfill M, Palazon J, Moyano E (2020) Transfecting Taxus x media protoplasts to study transcription factors BIS2 and TSAR2 as activators of taxane-related genes. Plant and Cell Physiology 61(3): 576–583. doi:10.1093/pcp/pcz225.

 

4. Sanchez-Muñoz R, Perez-Mata E, Almagro L, Cusido RM, Bonfill M, Palazon J, Moyano E (2020) A novel hydroxylation step in the taxane biosynthetic pathway: A new approach to paclitaxel production by synthetic biology. Frontiers in Bioengineering and Biotechnology 8 (410): 1-14.

doi: 10.3389/fbioe.2020.00410 (IF 5,122)

 

5. Hernández-Altamirano JM, Ugidos UF, Palazón J, Bonfill M, García-Angulo P, Álvarez J, Acebes JL, Bye R, Encina A (2020) Production of encecalin in cell cultures and hairy roots of Helianthella quinquenervis (Hook.) A. Gray. Molecules 25: 3231-3251. doi:10.3390/molecules25143231 (IF 3,060)

 

6. Escrich A, Almagro L, Moyano E, Cusido RM, Bonfill M, Hosseini B, Palazon J (2021) Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes. Plant Physiology and Biochemistry 163: 68-75. doi:10.1016/j.plaphy.2021.03.047 (IF 3,720)

 

7. El-Garhy HAS, Sherif HSA, Soliman SM, Haredy SA, Bonfill M (2021) Effect of gamma rays and colchicine on silymarin production in cell suspension cultures of Silybum marianum: A transcriptomic study of key genes involved in the biosynthetic pathway. Gene 790: 145700 (IF 3,688)

doi:10.1016/j.gene.2021.145700

 

8. Alcalde MA, Cusido RM, Moyano E, Palazon J, Bonfill M (2022) Metabolic gene expression and centelloside production in elicited Centella asiatica hairy root cultures. Industrial crops and products 184: 114988. doi.org/10.1016/j.indcrop.2022.114988 (IF 5.645)

 

9. Selim DA, Zayed M, Ali MM, Eldesouky HS, Bonfill M, El-Tahan A, Ibrahim OM, El-Saadony MT, El-Tarabily KA, AbuQamar SF, Elokkiah S (2022) Germination, physio-anatomical behavior, and productivity of wheat plants irrigated with magnetically treated seawater. Frontiers in Plant Sciences

doi: 10.3389/fpls.2022.923872

 

10. Escrich A, Cusido RM, Bonfill M, Palazon J, Sanchez-Muñoz R, Moyano E (2022) The epigenetic regulation in plant specialized metabolism: DNA methylation limits paclitaxel in vitro biotechnological production. Frontiers in Plant Sciences 13: 1-12. doi: 10.3389/fpls.2022.899444 (IF 6.627)

 

11. Alcalde MA, Müller M, Munné-Bosch S, Landín M, Gallego PP, Bonfill M, Palazon J, Hidalgo-Martinez D (2022) Using machine learning to link the influence of transferred Agrobacterium rhizogenes genes to the hormone profile and morphological traits in Centella asiatica hairy roots. Frontiers in Plant Sciences doi: 10.3389/fpls.2022.1001023 (IF 6.627)

 

12. Perez-Matas E, Hanano A, Moyano E, Bonfill M, Cusido RM, Palazon J. (2022) Insights into the control of taxane metabolism: Molecular, cellular, and metabolic changes induced by elicitation in Taxus baccata cell suspensions. Frontiers in Plant Science 13: 942433. doi: 10.3389/fpls.2022.942433

 

13. Perez-Matas E, Garcia-Perez P, Bonfill M, Lucini L, Hidalgo-Martinez D, Palazon J. (2023) Impact of Elicitation on Plant Antioxidants Production in Taxus Cell Cultures. Antioxidants 12: 887.

doi: 10.3390/antiox12040887

 

14. Escrich A, Palazon J, Sanchez-Muñoz R, Hidalgo-Martinez D, Bonfill M, Moyano E (2023) Polyploidy as a strategy to increase taxane production in yew cell cultures: Obtaining and characterizing a Taxus baccata tetraploid cell line. Plant Science

 

Reviews (last 5 years)                         

1. Sanchez-Muñoz R, Moyano E, Khojasteh A, Bonfill M, Cusido RM, Palazon J (2019) Genomic methylation in plant cell cultures: A barrier to the development of commercial long-term biofactories. Engineering in Life Sciences 19:872-879. DOI: 10.1002/elsc.201900024

 

2. Alcalde MA, Perez-Matas E, Escrich A, Cusido RM, Palazon J, Bonfill M (2022) Biotic elicitors in adventitious and hairy root cultures: a review from 2010 to 2022. Molecules

doi.org/10.3390/molecules27165253

 

3. Perez-Matas E, Hidalgo-Martínez D, Escrich A, Alcalde MA, Moyano E, Bonfill M, Palazon J. (2023) Genetic approaches in improving biotechnological production of taxane: An update. Frontiers in Plant Science DOI 10.3389/fpls.2023.1100228 (IF 6,627)

 

 

CURRENT PROJECTS

 

Title: Plant factories for the production of anticancer compounds: deepening the knowledge of its biosynthesis, regulation and red-ox homeostasis under elicitation conditions. Ministry of Economy and Competitiveness (PID2020-113438RB-I00). 2021-2024.

Title: Sustainable cultivation of Corema album berries in the Doñana area and its impacts on human health (COREBERRY). Proyecto líneas estratégicas MCIN /AEI / Unión Europea NextGenerationEU / PRTR (PLEC2022-009299). 2023-2025

Title: Natural Products and Plant Biotecnology. Cosolidated Research Group SGR2021-00693. Generalitat de Catalunya. 2022-2025