Biomedical Laboratory and Instrumentation

Index

General Information

Estimated learning time

Learning objectives

Teaching blocks

Official assessment of learning outcomes

Reading and study resources

General Information

Course unit nameBiomedical Laboratory and Instrumentation
Course unit code365367
Academic year2024/2025
CoordinatorJosep Ignasi Ramirez Sunyer
DepartmentDepartment of Biochemistry and Molecular Biomedicine
Credits6
Single programS

Estimated learning time

Total number of hours : 150 Hours
ActivitiesType of trainingHoursObservations
Face-to-face and/or online activities59
- Lecture Face-to-face 19
- IT-based class Face-to-face 8
- Laboratory session Face-to-face 32
Supervised project40
Independent learning51

Learning objectives

Referring to knowledge

The learning objectives and skills of this course are dependent on what was taught in the first semester and what has been acquired so far in the second semester. This explains why the course is delivered in close coordination with other courses that the students have completed or are currently studying, namely: Biophysics, Chemistry, Biochemistry, and Cytology.

The general objective of this course is to ensure students acquire the skills required to work in a research laboratory in the field of chemistry or biology.

 

Referring to knowledge

  • Know the theoretical and functional foundations of the most frequently used instrumentation in an analytical or research laboratory and recognize the purposes they serve.
  • Be clear about the difference between qualitative and quantitative analyses and the adaptation of instrumentation to each type of procedure.
  • Theoretical principles of separation by centrifugation, filtration, chromatography, electrophoresis.
  • Theoretical principles of the identification and/or quantification of compounds by spectroscopy and spectrometry. Bases of absorbance, fluorescence, and resonance.
  • Learn the basics of optical and electron microscopy.
  • Know the most useful databases in scientific research.

Referring to skill acquisition

  • Be familiar with the safety measures and hygiene standards required to work in a research laboratory.
  • Know how to use the basic instrumentation of a laboratory (pipettes, Teflon homogenizers, extractor hoods, benchtop centrifuges, electrophoresis trays, separation columns, etc.).
  • Know how to calculate dilutions and develop simple chemical solutions.
  • Know how to quantify a compound from a standard and a pattern.
  • Know how to use Excel as a numerical representation tool.
  • Learn to use the light microscope correctly.
  • Learn how to perform a protein electrophoresis.
  • Know how to use information and bibliography databases.
  • Acquire notions of how to write a scientific paper and present the results in public.
  • Learn to plan a simple experiment or analysis with the right instrumentation.

Learning objectives

Referring to knowledge

The learning objectives and skills of this course are dependent on what was taught in the first semester and what has been acquired so far in the second semester. This explains why the course is delivered in close coordination with other courses that the students have completed or are currently studying, namely: Biophysics, Chemistry, Biochemistry, and Cytology.

The general objective of this course is to ensure students acquire the skills required to work in a research laboratory in the field of chemistry or biology.

 

Referring to knowledge

  • Know the theoretical and functional foundations of the most frequently used instrumentation in an analytical or research laboratory and recognize the purposes they serve.
  • Be clear about the difference between qualitative and quantitative analyses and the adaptation of instrumentation to each type of procedure.
  • Theoretical principles of separation by centrifugation, filtration, chromatography, electrophoresis.
  • Theoretical principles of the identification and/or quantification of compounds by spectroscopy and spectrometry. Bases of absorbance, fluorescence, and resonance.
  • Learn the basics of optical and electron microscopy.
  • Know the most useful databases in scientific research.

Referring to skill acquisition

  • Be familiar with the safety measures and hygiene standards required to work in a research laboratory.
  • Know how to use the basic instrumentation of a laboratory (pipettes, Teflon homogenizers, extractor hoods, benchtop centrifuges, electrophoresis trays, separation columns, etc.).
  • Know how to calculate dilutions and develop simple chemical solutions.
  • Know how to quantify a compound from a standard and a pattern.
  • Know how to use Excel as a numerical representation tool.
  • Learn to use the light microscope correctly.
  • Learn how to perform a protein electrophoresis.
  • Know how to use information and bibliography databases.
  • Acquire notions of how to write a scientific paper and present the results in public.
  • Learn to plan a simple experiment or analysis with the right instrumentation.

Teaching blocks

  • 1 Basic laboratory techniques

  • 2 Identification and quantification

  • 3 Spectroscopic techniques

  • 4 Basic microscopy techniques

  • 5 Advanced analytical techniques

  • 6 Macromolecule and cell analysis techniques

  • 7 Documentation techniques

Official assessment of learning outcomes

This subject, given its practical nature and in agreement with the Board of Studies, only provides for the possibility of continuous assessment, which includes:
Mandatory attendance at all theory classes, practicals, computer sessions and scientific communication seminars (unjustified absences are only accepted for practical sessions).
Evaluation of all additional material requested: questionnaires, lab notebooks, reports or similar.
Evaluation of different written tests during the teaching period.
The weighting of each part of the subject is as follows:

Analysis laboratory: 20%.
Observation laboratory: 20%.
Bioinformatics: 10%.
Chemical laboratory: 50%.
This scoring system is an estimate and can be modified based on the specific teaching and organizational requirements of each campus. In any case, it will always be defined before the start of the corresponding course.

 

Qualification criteria

The pass is achieved with a minimum overall grade of 50% after adding the weighted scores obtained in each part, provided that the written test of the chemical laboratory reaches at least 40% of the weighted grade that corresponds to it. In case of not reaching 40% of the weighted grade, the written test of the chemical laboratory will be recoverable in the reassessment exam.

Examination-based assessment

The student has the right to request a single assessment, during the first week of the course, as long as he has taken and passed the tests of the practical classes beforehand.

Reading and study resources

Book

Bioquímica: Técnicas y Métodos, P. Roca, J. Oliver, AM. Rodríguez, Ed. Hélice - 2003, ISBN 978-84-921124-8-7.

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Book

Clinical Biochemistry: Techniques and Instrumentation. JS Varcoe, World Scientific-2001, ISBN 978-981-02-4556-6

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Book

Textbook for Biomedical Instrumentation. KW. Scott, New Delhi : CBS Publishers & Distributors, 2007

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Book

An Introduction to Microscopy. S Bell, K Morris. CRC Press - 2009, ISBN 9781420084504.

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Book

Introduction to Electron Microscopy for Biologists, Volume 88: Methods in Cell Biology by Terry D. Allen Ph.D. D.Sc. Elsevier Inc. 2008. ISBN: 0123743206.

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Online access restricted to UB users.

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Book

Introducción a la bioinformática. T.K. Attwood, D. J. Parry-Smith, Prentice Hall, 2002, ISBN: 8420535516.

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Book

Principios de Química. P. Atkins, L. Jones, 5rd ed. Editorial Panamericana, 2012, ISBN: 9789500602822

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Iniciació a les tècniques histològiques vegetals i animals i nota sobre les tècniques de transparentat. Institut d’Estudis Catalans, 2006, ISBN: 8472838838.

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