Dr. Judit Castillo is a Serra Hunter Lecturer Teaching position at the Genetics Unit, Department of Biomedicine, University of Barelona, and is a member of the Molecular Biology of Reproduction and Develompment research group (IDIBAPS).
Previous positions:
-Sara Borrell Postdoctoral Fellowship from the Spanish Ministry of Economy and Competitiveness (Ministerio de Economia y Competitividad, Acción Estratégica en Salud, CD17/00109).
-Board member and treasurer of the Network for Young Researchers in Andrology (NYRA).
- MSCA Postdoctoral Researcher in Lead Pharma Holding BV (Nijmegen, The Netherlands), within the Marie SklodowscaCurie ITN GrowSperm.
- PhD student in the Molecular Biology of Reproduction and Development Group, with the thesis project: Characterization of the sperm nuclear protein content and its epigenetic potential.
Main Judit's publications:
Publications
26: de la Iglesia A, Jodar M, Oliva R, Castillo J.
Insights into the sperm chromatin and implications for male infertility from a protein perspective.
WIREs Mech Dis. 2023 Mar;15(2):e1588
25: Castillo J, de la Iglesia A, Leiva M, Jodar M, Oliva R.
Proteomics of human spermatozoa.
Hum Reprod. 2023 Aug 25:dead170
24: Barrachina F, de la Iglesia A, Jodar M, Soler-Ventura A, Mallofré C, Rodriguez-Carunchio L, Goudarzi A, Corral JM, Ballescà JL, Castillo J, Oliva R.
Histone H4 acetylation is dysregulated in active seminiferous tubules adjacent to testicular tumours.
Hum Reprod 2022;37:1712-1726
23: de la Iglesia A, Jauregi P, Jodar M, Barrachina F, Ded L, Mallofré C, Rodríguez-Carunchio L, Corral JM, Ballescà JL, Komrskova K, Castillo J, Oliva R.
H4K5 butyrylation coexist with acetylation during human spermiogenesis and are retained in the mature sperm chroma-tin.
Int J Mol Sci 2022;23:12398.
22: Barrachina F, Battistone MA, Castillo J, Mallofré C, Jodar M, Breton S, Oliva R.
Sperm acquire epididymis-derived proteins through epididymosomes.
Hum Reprod. 2022; 37:651-668
21: Gianluca Arauz-Garofalo, Meritxell Jodar, 3, Mar Vilanova, Alberto de la Iglesia Rodriguez, Judit Castillo, Ada Soler-Ventura, Rafael Oliva, Marta Vilaseca, Marina Gay.
Protamine characterization by top-down proteomics: Boosting proteoform identification with DBSCAN.
Proteomes 2021, 9, 21. https://doi.org/10.3390/proteomes9020021
20: Soler-Ventura A, Gay M, Jodar M, Vilanova M, Castillo J, Arauz-Garofalo G, Villarreal L, Ballescà JL, Vilaseca M, Oliva R.
Characterization of human sperm protamine proteoforms through a combination of top-down and bottom-up mass spectrometry approaches.
Journal of Proteome Research. 2020 19(1):221-237. doi: 10.1021/acs.jproteome.9b00499
19: Castillo J, Bogle OA, Jodar M, Torabi F, Delgado-Dueñas D, Estanyol JM, Ballescà JL, Miller D, Oliva R.
Proteomic changes in human sperm during sequential in vitro capacitation and acrosome reaction.
Frontiers in Cell and Develop Biology. 2019. 20;7:295. doi: 10.3389/fcell.2019.00295
18: Castillo J, Jodar M, Oliva R.
The contribution of human sperm proteins to the development and epigenome of the preimplantation embryo.
Hum Reprod Update 2018; 24, 535-555
17: Soler-Ventura A, Castillo J, de la Iglesia A, Jodar M, Barrachina F, Ballesca JL, Oliva R.
A Step-By-Step Protocol For Mammalian Sperm Protamine Extraction And Analysis.
Protein Pept Lett
16: Castillo J, Estanyol JM, Ballescá JL, Oliva R.
Human sperm chromatin epigenetic potential: genomics, proteomics, and male infertility.
Asian J Androl. 2015;17:601-9.
15: Vilagran I, Yeste M, Sancho S, Castillo J, Oliva R, Bonet S.
Comparative analysis of boar seminal plasma proteome from different freezability ejaculates and identification of Fibronectin 1 as sperm freezability marker.
Andrology. 2015;3:345-56.
14: Zatecka E, Castillo J, Elzeinova F, Kubatova A, Ded L, Peknicova J, Oliva R.
The effect of tetrabromobisphenol A on protamine content and DNA integrity in mouse spermatozoa.
Andrology. 2014;2:910-7.
13: Azpiazu R, Amaral A, Castillo J, Estanyol JM, Guimerà M, Ballescà JL, Balasch J, Oliva R.
High-throughput sperm differential proteomics suggests that epigenetic alterations contribute to failed assisted reproduction.
Hum Reprod. 2014;29:1225-37.
12: Castillo J, Amaral A, Oliva R.
Sperm nuclear proteome and its epigenetic potential.
Andrology. 2014;2:326-38.
11: Amaral A, Castillo J, Ramalho-Santos J, Oliva R.
The combined human sperm proteome: cellular pathways and implications for basic and clinical science.
Hum Reprod Update. 2014;20:40-62.
10: Vilagran I, Castillo J, Bonet S, Sancho S, Yeste M, Estanyol JM, Oliva R.
Acrosin-binding protein (ACRBP) and triosephosphate isomerase (TPI) are good markers to predict boar sperm freezing capacity.
Theriogenology. 2013;80:443-50.
9: Amaral A, Castillo J, Estanyol JM, Ballesca JL, Ramalho-Santos J, Oliva R.
Human sperm tail proteome suggests new endogenous metabolic pathways.
Mol Cell Proteomics. 2013 Feb;12(2):330-42.
8: Jodar M, Kalko S, Castillo J, Ballescà JL, Oliva R.
Differential RNAs in the sperm cells of asthenozoospermic patients.
Hum Reprod. 2012;27:1431-8.
7: Simon L, Castillo J, Oliva R, Lewis SE.
Relationships between human sperm protamines, DNA damage and assisted reproduction outcomes.
Reprod Biomed Online. 2011;23:724-34.
6: de Mateo S, Castillo J, Estanyol JM, Ballescà JL, Oliva R.
Proteomic characterization of the human sperm nucleus.
Proteomics. 2011;11:2714-26.
5: Castillo J, Simon L, de Mateo S, Lewis S, Oliva R.
Protamine/DNA Ratios and DNA Damage in Native and Density Gradient Centrifuged Sperm From Infertile Patients.
J Androl. 2011;32:324-32.
4: Oliva R, Castillo J.
Proteomics and the genetics of sperm chromatin condensation.
Asian J Androl. 2011;13:24-30.
3: Oliva R, De Mateo S, Castillo J, Azpiazu R, Oriola J, Ballescà JL.
Methodological advances in sperm proteomics.
Hum Fertil (Camb). 2010 Dec;13(4):263-7
2: Jodar M, Oriola J, Mestre G, Castillo J, Giwercman A, Vidal-Taboada JM, Ballescà JL, Oliva R.
Polymorphisms, haplotypes and mutations in the protamine 1 and 2 genes.
Int J Androl. 2011;34:470-85
1: Barrachina F, Anastasiadi D, Jodar M, Castillo J, Estanyol JM, Pifarrer F, Oliva R.
Identification of a complex population of chromatin-associated proteins in addition to protamines in the European sea bass (Dicentrarchus labrax) sperm.
Systems Biology in Reproductive Medicine 2018; 64:502-517